Department of Biology, Education College, Salahaddin University, Erbil, Iraq


Objective: This study aimed to isolate bacteria from the eyelids of that non-cosmetic user group and eye cosmetic user group. Materials and Methods: The study samples were taken by swabs, and the bacterial isolates were identified by Vitek2 compact system, and the antibacterial activity of Kohl (Al-Ethmed) was performed by well diffusion technique. Results: The highest percentage of bacterial isolates was gram positive Staphylococcus. aureus (21.40%) followed by Coagulase negative Staphylococcus (CNS) (19.60%) and - hemolytic Streptococcus (16.10%), while the percentage of gram negative isolates represented by Echerichia coli, Klebsiella Spp. and Proteus Spp. was (3.60%) and the percentage of Pseudomonas aeruginosa was (1.80%). The percentage of most bacterial isolates increased within cosmetic user group. The geographical areas showed that there was some difference in percentage of the species between Erbil, Sulaymania and Kirkuk. According to the ages, -hemolytic Streptococcus were shown to be the common isolates among all age groups, while according to gender; the results revealed that S. aureus (21.20%), CNS (15.10%), Haemophilus (9%), E. coli and Proteus (6.60%) and p. aeruginosa (3%) were the predominant isolates in females. However, -hemolytics Streptococcus (18.10%), -hemolytic Streptococcus (22.70%) were of the highest percentage in males followed by Micrococcus luteus (13.60%), S.pyogenes (9%) and Klebseilla (4.50%). The results showed that S. aureus, -hemolytic Streptococcus and -hemolytic Streptococcus were resistant to (CT) with a percentage of (71.4%), (75%), (66.6%), (60%) and (84%) respectively, while E. coli, Klebseilla species, p. aeruginosa and Proteus showed (100%) resistance to cefuroxime (CX). It is worth mentioning from our study that Kohl (which Prophet Mohammed (Peace be upon him) used it and recommended to use) as cosmetics exhibited considerable antibacterial activities, when the different kohl concentrations were used inhibition of the growth of both -hemolytic Streptococcus and p. aeruginosa was achieved at all concentrations. However, CNS, Haemophilus and Klebseilla isolates were inhibited at (500), (2500) and (62.5) µg/ml.